Figure 2

Serial dilution experiments showing the sensitivity of the alternative techniques for K-Ras mutation detection: hybridisation assay without PNA (A) and PNA-clamped probe assay (B). DNA from A549 (lung adenocarcinoma) cell line, which harbours an homozygous K-Ras mutation (G12S), was diluted into DNA from a wild-type K-Ras HT29 cell line.