Table 2 DNA sequences of primers, PNA oligomer, and probes for detecting K-Ras mutations

From: Detection of K-Ras mutations in tumour samples of patients with non-small cell lung cancer using PNA-mediated PCR clamping

Name

DNA (5′–3′) or PNA (NH 2 -CONH 2 ) sequence a

Position, b nt

K-Ras Fc

GGAGTATTTGATAGTGTATTAACCT

9–33

K-Ras R I

AGAATGGTCCTGCACC

251–236

K-Ras R II

GTCCTGCACCAGTAATATGC

244–226

Anchor

LC-Red 640-ACTACCACA AGTTTATATTCAGTCATTTTCAGCAGG-Ph

121–86

Sensor

CCTACGCCACCAGCTC-Flu

138–124

PNA

CCTACGCCACCAGCTCC

138–122

  1. aThe 3′ end of the anchor probe was phosphorylated to prevent probe elongation by Taq polymerase during PCR. Underlined, nucleotide complementary to wild-type bases of codon 12 and 13.
  2. bThe base numbering is according to GenBank accession no L00045.
  3. cF=forward; R=reverse; Flu=fluorescein; LC-Red=LightCycler-Red; Ph=phosphorylated; R I: K-Ras reverse primer used for Light-cycler analysis and classical sequencing; R II: K-Ras reverse primer used for sequencing of PNA–PCR products.
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