Figure 5 | British Journal of Cancer

Figure 5

From: Association of constitutively activated hepatocyte growth factor receptor (Met) with resistance to a dual EGFR/Her2 inhibitor in non-small-cell lung cancer cells

Figure 5

Met shRNA downregulates ErbB family receptor activation. Whole cell extracts were made from control (H441-pLKO.1) and Met shRNA (H441-Met shRNA) cells. (A) Whole cell extracts (200 μg) were analysed on each RTK array membrane, and activation status of receptors was assessed using antiphosphotyrosine antibody and numbered as in Figure 1A. 1: EGFR; 2: Her2; 3: Her3; 6: Met; 7: MSRP; and 9: Ret. (B) The cell extracts analysed in panel A were analysed by immunoblotting with indicated antibodies. β-Tubulin was used as a loading control. (C) Cell extracts (500 μg) used in panel A were subjected to immunoprecipitation using either anti-Her2 or anti-Her3 antibodies followed by immunoblotting with indicated antibodies. IgG was used as a negative control.

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