Figure 5
From: MicroRNA deregulation and pathway alterations in nasopharyngeal carcinoma
Inverse correlation between cyclin E2 expression with miR-34c, miR-200a, and miR-9 in NPC. (A) Predicted binding sites for miR-34c-5p, miR-200a, and miR-9 on the 3′UTR of cyclin E2. (B) Expression levels of miR-34c-5p, miR-200a, and miR-9 in normal and NPC tissues. (C) Correlation analysis between cyclin E2 and three microRNAs. Pearson's correlation coefficient and P-value for individual analysis are shown in the inserts. (D) Quantification of miR-9 in HK1 cells after transduction with either negative control lentivirus (HK1/neg) or lentivirus encoding miR-9 (HK1/miR-9). Levels of miR-9 and miR-16 were expressed as 39 – Ct. (E) Quantification of CCNE2 and β-2-microglobulin (B2M) transcripts in HK1/neg and HK1/miR-9 cells. Results are mean±s.d. of three independent experiments. A significant reduction in CCNE2 level was observed after miR-9 expression (HK1/neg vs HK1/miR-9, CCNE2: P=0.014, B2M: P=0.258). (F) Reduced expression of CCNE2 protein in HK1/miR-9 cells.
