Figure 5

CXCR1 and CXCR2 overexpression enhances cell motility and invasion. SBC-2 and A375P cells expressing CXCR1 or CXCR2 were seeded on non-coated or Matrigel-coated membranes for motility (A and B) and invasion (C and D) assays, respectively, and incubated for 24 h. Serum-free medium containing 10 ng ml⁻¹ CXCL-8 was added to the lower chamber. The cells that did not migrate through the Matrigel and/or pores in the membrane were removed, and cells on the other side of the membrane were stained and photographed at 200 × magnification. Cells were counted in 10 random fields (200 × ) and expressed as the average number of cells per field of view. The values are number of migrated cells±s.e.m. This is a representative of three experiments done in triplicate. ^*Significantly different from control CXCL-8-treated cells (P<0.05).