Figure 2

Expression of hnRNP K associated with NM in PCa. (A) Representative 1D WB analysis of NM isolated from NHP, NT and PCa with different Gleason scores. The relative molecular weights of hnRNP K and the splicing variants J are reported on the left. (B) The comparison among the relative amounts of hnRNP K as determined by quantitative analysis of four NHP, 24 NT and 27 PCa samples with Gleason score 4–7 and 20 PCa samples with Gleason score 8–9. The ordinates represent the mean±s.e. (reported also in the inset). The increase in hnRNP K expression was found to be significant between NT and PCa with Gleason score 4–7 (P=0.014) and highly significant between Gleason score 4–7 and 8–9 (P=0.008). (C) Scatterplots of relative amounts of hnRNP K within the NM isolated from NT and PCa samples. Horizontal lines indicate the mean values. (D) Biochemical (PSA) progression-free survival according to hnRNP K expression within the NM. The dotted curve denotes patients (n=31) whose tumours had low hnRNP K expression (⩽0.60) and the solid curve denotes those (n=16) with high expression (>0.60); P=0.032.