Figure 3

Representative 2D gel maps of the NM proteins extracted from NT (A) and PCa (B) tissues and from LNCaP (E) and PC3 (F) cell lines. Tumour-associated proteins are marked by circles and identified with the same number used in Boccardo et al (2003); silver-stained gels. The proteins resolved in 2D were transferred to Hybond-P membranes and probed with anti-hnRNP K antibody. Representative 2D WB of NM proteins extracted from NT (C), PCa (D), LNCaP and PC3 cells in the absence (G and H, respectively) and presence of 10 μ M BIC for 72 h (I and J, respectively). The circles in D, G, H, I and J highlight the NM-6 spot that corresponds to an acidic isoform of hnRNP K. This isoform is absent in NT (C) and is downexpressed in LNCaP cells after treatment with BIC (I). The relative molecular weights of standard proteins are reported on the left.