Figure 4

(A, B) Confocal microscopy analyses carried out on control LNCaP cells clearly show strong hnRNP K staining in the nucleus and lower staining in the cytoplasm. After exposure to 10 μ M BIC for 72 h, the staining of the cytoplasm disappears and the staining in the nucleus gets fainter. Cells were immunostained with anti-hnRNP K antibody (green) and DNA was visualised with SYTOX Orange (blue). Images from control (Ctrl) and treated (BIC) cells were acquired with identical acquisition settings and representative cells were photographed at low magnification (A; scale bars: 25 μm) and at high magnification (B; scale bars: 10 μm). (C, D) Comparison between hnRNP K expression, in the different cellular compartments, in control and treated cells. Representative 1D WB (C); the relative molecular weights are indicated on the left. The ordinates in (D) represent the mean±s.e. (reported also in the inset) of the relative amounts of hnRNP K, as determined by quantitative analysis, of four BIC treatment experiments and from four to seven different western blots, as in some samples were run on two different gels. When the cells were exposed to BIC, the expression of hnRNP K underwent an overall decrease. This decrease was significant within the NM (P=0.019).