Figure 2 | British Journal of Cancer

Figure 2

From: E. coli NfsA: an alternative nitroreductase for prodrug activation gene therapy in combination with CB1954

Figure 2

In vitro kinetics of CB1954 activation by purified NfsA and NfsB, using NADH or NADPH as cofactors. Graphs plot initial rate of CB1954 activation (v) normalised by enzyme concentration (E), using 50 μ M NAD(P)H at a range of CB1954 concentrations, with Michaelis–Menten curves fitted to the data. (A) Kinetics of NfsA. (B) Kinetics of NfsB (solid lines); for comparison, the dotted line again shows the kinetics of NfsA using NADPH.

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