Table 2 Antibodies used for the immunohistochemical study

From: High Ki67, Bax, and thymidylate synthase expression well correlates with response to chemoradiation therapy in locally advanced rectal cancers: proposal of a logistic model for prediction

Antibody

Clone

Source

Dilution

Antigen retrieval

Ki67

Monoclonal MIB-1

DakoCytomation, Glostrup, Denmark

1 : 50

Treatment in hot bath (95–98°C) for 40 min (Dako Target Retrieval Solution (pH 9.0))

Mcm3

Monoclonal 3A2

MBL, Nagoya, Japan

1 : 400

Microwave treatment for 15 min (Dako Target Retrieval Solution (pH 9.0))

Bax

Monoclonal B-9

Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA

1 : 100

Microwave treatment for 15 min (citrate buffer (pH 6.0, 0.01 mol/l))

Bcl-2

Monoclonal 124

DakoCytomation

1 : 50

Microwave treatment for 15 min (Dako Target Retrieval Solution (pH 9.0))

ssDNA

Polyclonal A4506

DakoCytomation

1 : 400

Not applied

Grp78

Polyclonal H-129

Santa Cruz Biotechnology, Inc.

1 : 100

Microwave treatment for 30 min (citrate buffer (pH 6.0, 0.01 mol/l))

TS

Monoclonal TS106

DakoCytomation

1 : 50

Autoclave (121°C) treatment for 15 min (citrate buffer (pH 6.0, 0.01 mol/l))

DPD

Polyclonal RDPDPA

Taiho Pharmaceuticals Co., Ltd., Tokyo, Japan

1 : 400

Autoclave (121°C) treatment for 10 min (EDTA (pH 8.0, 1 mmol/l))

CD34

Monoclonal QBEnd 10

DakoCytomation

1 : 500

Not applied

VEGF

Polyclonal A-20

Santa Cruz Biotechnology, Inc.

1 : 100

Not applied

Nestin

Polyclonal N1602

IBL, Takasaki, Japan

1 : 500

Not applied

LAT1

Monoclonal

Fuji Biomedix, Tokyo, Japan

Prediluted

Microwave treatment for 5 min (in the buffer supplied by kit)

  1. Mcm, minichromosome maintenance; Grp78, glucose-regulated protein 78; TS, thymidylate synthase; DPD, dihydropyrimidine dehydrogenase; VEGF, vascular endothelial growth factor; LAT1, L-type amino-acid transporter 1.
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