Figure 1 | British Journal of Cancer

Figure 1

From: Expression of a protease-resistant insulin-like growth factor-binding protein-4 inhibits tumour growth in a murine model of breast cancer

Figure 1

Expression of IGF1R, IGFBP4 and the IGFBP4 protease, PAPP-A. (A) Western blot analysis of IGF 1Rβ in 4T1.2 cell lysate (lane 1). Cell lysate from MCF7 cells is shown in lane 2 as positive control. Positions of molecular weight markers are indicated (kDa). (B) Western blot analysis of IGFBP4 expression in cell lysate (lane 1) and conditioned medium (lane 2) from 4T1.2 cells. Recombinant human IGFBP4 (10 ng) is shown in lane 3 as a positive control. Positions of molecular weight markers are indicated (kDa). (C) Western blot analysis of IGFBP4 in normal mammary fat pad (lane 3) and 4T1.2 mammary fat pad tumours (lanes 4 and 5). IGFBP4 expression in cell lysate (lane 1) and conditioned medium (lane 2) from 4T1.2 cells is shown for reference. Positions of molecular weight markers are indicated (kDa). (D) RT–PCR of PAPP-A. PAPP-A (top panel) was amplified by RT–PCR from cDNA prepared from the 4T1.2 mammary fat pad tumours (lanes 1, 2 and 3), 4T1.2 cells (lane 4) and MC3T3 mouse osteoblasts (lane 5). Molecular weight markers (1 Kb ladder) are shown in lane 6. β-Actin (lower panel) was amplified from all samples to control for sample quality.

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