Figure 1
From: Regulation of the apoptosis-inducing kinase DRAK2 by cyclooxygenase-2 in colorectal cancer
Regulation of HCA7 cell viability and apoptosis by COX-2 inhibition. (A) Representative examples of the distribution of staining for FITC–Annexin V (x axis, FL1) and propidium iodide (y axis, FL3) in HCA7 cells treated for 24 h with vehicle control (top), SC236 (5 μ M, middle) and SC236 (5 μ M)/PGE2 (1 μ M, bottom). (B) Quantification of cell viability and apoptosis. Viable cells (upper chart) and cells in early stages of apoptosis showing positive Annexin V staining but negative for propidium iodide (lower chart), represented as a percentage of the total cell population. Values shown are mean percentages±s.e.m. of total cell counts across replicate experiments (n=4); *P<0.05, **P<0.01; one-way ANOVA. Each replicate involved the counting of a total of a minimum of 10 000 events.
