Figure 2
Expression of Dicer and EMT markers in a human mammary cancer progression cellular model. Expression of Dicer in the human breast tumour progression cellular model: (A) western blot analysis of Dicer expression in the four human breast cell lines, namely HMEC, HMEC+hTERT, HMEC+LT+hTERT, and HMLER (expressing H-rasV12) cells. The signal intensity of Dicer was normalised to that of actin. (B)The relative levels of Dicer mRNA were measured by real-time RT–PCR, and each bar represents the mean±s.d. of the PCRs in triplicate (▪). Dicer protein levels were quantified using Quantity One software (BioRad, Marnes-la-Coquette, France) and expressed as protein relative quantity. The ratios of Dicer/actin of three independent studies were expressed as mean±s.d. (
). Morphological characteristics and expression of epithelial and mesenchymal markers in the human breast tumour progression cellular model: (C) Morphological characteristics of the HMEC+hTERT, HMEC+LT+hTERT, and HMLER cells in vitro as assessed by phase-contrast microscopy. Images are shown at × 40 magnification. (D) Western blot analysis of E-cadherin and vimentin expression in the four human breast cell lines, HMEC, HMEC+hTERT, HMEC+LT+hTERT, and HMLER. Actin served as a loading control. E, epithelial phenotype; M, mesenchymal phenotype.
