Figure 4

Effect of the IMiDs on the expression of metastatic markers and intracellular signalling proteins. CT26 cells were cultured with thalidomide (tha), lenalidomide (lena) or pomalidomide (poma) (0–30 μ M) for 3 days. The expressions of VEGF-R1, E-cadherin and MMP-9 were assessed by immunoblot analyses. Representative blots are shown (A), and densitometric analysis using GAPDH as the loading control was carried out (B). The levels of VEGF within the supernatant were also assessed by ELISA (C). The expressions of key signalling proteins after treatment (10 μ M – designated by a ‘T’) were assessed and compared with controls with no treatment (‘C’) and those with equal DMSO solvent exposure (‘D’) (D). Each data point is the mean and s.d. of three separate blots.