Figure 5

Effect of the IMiDs on the expression of angiogenic and metastatic markers. CT26 cells were cultured with the drugs for 3 days, and the growth medium was harvested. These tumour-conditioned media were subjected to assessment of proteins associated with angiogenesis and metastasis. Assessing conditioned media derived from untreated tumours indicated that some cytokines were below the magnitude background (BK) (A, B). Three cytokines, VEGF, IP10 and TIMP1, that were registered as consistently present in the media were assessed after treatment and results show that only VEGF was affected (C).