Figure 5

Nuclear factor of activated T-cells (NFAT) regulates cyclooxygenase-2 (COX-2) promoter activity in melanoma cell lines. (A) Schematic of the human COX-2 promoter and the COX-2 luciferase reporter vectors used. Cells were transfected with the panel of reporter vectors and treated 24 h later with TPA (50 nM)/ionomycin (iono) (1 μ M) for 4 h. Reporter activity is shown for A375 cells (B) and WM266-4 cells (C). Values are means from three independent experiments performed in triplicate (± s.e.m.). Statistical analysis performed by one-way ANOVA, ^**P⩽0.01, ^*** P⩽0.001 vs control vector containing all functional NFAT binding sites ($).