Table 1 Primers used for RT–PCR detection of aromatase, aromatase promoter regions pI.3, pI.4, and pII, cyclooxygenases 1 and 2 and S14 (control)

From: Melatonin inhibits aromatase promoter expression by regulating cyclooxygenases expression and activity in breast cancer cells

mRNA

Sequence

Aromatase (F)

5′–TATTGGAAATGCTGATTGCGG

Aromatase (R)

5′-TTGGGCTTGGGGAAATACTCG

pI.3 (F)

5′-GGGCTTCCTTGTTTTGACTGTAA

pI.3 (R)

5′-AGAGGGGGCAATTTAGAGTCTGTT

pII (F)

5′-CTCTGAAGCAACAGGAGCTATAGA

pII (R)

5′-CAGGCACGATGCTGGTGATG

pI.4 (F)

5′-AACGTGACCAACTGGAGCCTG

pI.4 (R)

5′-CATCACCAGCATCGTGCCTG

COX-1 (F)

5′-ACCCGCACGGGCTATTCCGGC

COX-1 (R)

5′-AGGCGCATGAGCATCTCTCGG

COX-2 (F)

5′-ATGTATGAGTGTGGGATTTGA

COX-2 (R)

5′-TCCAAAATCCCTTGAAGTGGG

S14 (F)

5′-TCACCGCCCTACACATCAAAC

S14 (R)

5′-TCCTGCGAGTGCTGTCAGAG

  1. (F) sense forward primer; (R) antisense (reverse) primer.
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