Figure 6
From: Gene silencing of HPV16 E6/E7 induced by promoter-targeting siRNA in SiHa cells
Detection of DNA methylation and H3K9 methylation of the HPV 16 E6/E7 promoter in SiHa cells treated by siRNA. (A) Sequence of pyrosequencing of the E6/E7 promoter. Genomic DNA was treated with sodium bisulphite to transfer unmethylated C to T, and to maintain methylated C as C. Therefore, in the results of pyrosequencing, T represents unmethylated C. Five CpGs in the promoter region were all unmethylated. (B) H3K9me3 expression detected by ChIP and real-time PCR. ΔΔC(t) were 0.86±0.13, 0.23±0.06 and 0.48±0.04 separately in the promoter–siRNA, negative control and blank control. The x-axes show different experiment groups, and y-axes show H3K9me3 expression, which was normalised to that of the negative control.
