Figure 4

Comparative ADCC activity of different cell types from a single healthy donor. (A) FACS analysis dot plot showing the enrichment of NK cells from PBMC (x-axis, CD56; y-axis, CD16) pre (left-hand dot pot) and post (right-hand dot plot) sorting. (B) NK ADCC activity. Graph of NK ADCC activity against SKCO-1 in the absence and presence of 20 μg ml⁻¹ of hPR1A3 at different effector : target ratios (x-axis) vs % cell lysis (y-axis). (C) FACS analysis plot showing the enrichment of monocytes from PBMC (x-axis, CD14 intensity; y-axis, number of events)- pre (left-hand histogram plot) and post (right-hand histogram plot) sorting. Grey line presents staining using CD14 antibody-PE; dark line represents staining with isotype antibody control-PE. (D) Monocyte ADCC activity. Graph of monocyte ADCC activity against SKCO-1, in the absence and presence of 20 μg ml⁻¹ of hPR1A3, at different effector : target ratios (x-axis) vs % cell lysis (y-axis). (E) FACS analysis plot showing the enrichment of granulocytes from fresh blood after sorting (x-axis, CD15 intensity; y-axis, frequency of events). Grey line presents staining using CD15 antibody-FITC; dark line represents staining with isotype antibody control-FITC. (F) Granulocyte ADCC activity. Graph of granulocyte ADCC activity against SKCO-1 in the absence and presence of 20 μg ml⁻¹ of hPR1A3, at different effector : target ratios (x-axis) vs % cell lysis (y-axis).