Table 1 Comparison of the ability of different hPR1A3 variants to elicit ADCP using the Ikoniscope

From: Humanised IgG1 antibody variants targeting membrane-bound carcinoembryonic antigen by antibody-dependent cellular cytotoxicity and phagocytosis

 

PMSA control

ghPR1A3

unPR1A3

Total targets

1336.00

1284.00

1428.00

Targets engulfed by macrophages

27.00

122.00

213.00

Conjugates between targets and macrophages

38.00

79.00

116.00

% of targets engulfed

2.02

9.50

14.92

% of targets conjugated

2.84

6.15

8.12

% phagocytosis+% conjugated

4.87

15.65

23.04

% ADCP using FACS

10.22

22.11

28.56

  1. The number of target cells that were engulfed by macrophages was counted directly using the Ikoniscope. Targets that were conjugated to macrophages were defined as having cell-to-cell membrane contact but no envelopment. Targets and macrophages could readily be identified separately. The percentages of engulfed and conjugated cells are compared with the percentage of targets classified as having undergone ADCP using FACS analysis of the same preparation of cells.
Back to article page