Figure 4

Effects of drug treatment on NF-κB activity in PC3 cells. (A) Bar graph illustrating the effects of 17-AAG and/or AZ10397767 administration on NF-κB transcriptional activity in PC3 cells. Drug-induced changes in NF-κB-driven luciferase activity were measured and adjusted, as previously described (legend to Figure 3), 24 h after addition of drugs. Values are expressed as the mean±s.e.m. of five separate experiments, ^**P<0.01. (B) Bar graph representing real-time PCR analysis of CXCL8 mRNA transcript levels in PC3 cells after 24 h exposure to 17-AAG (1 nM or 1 μ M). Cells were pre-treated for 4 h with 20 nM AZ10397767 or vehicle control to assess the effect on cellular IL-8 expression, used here as a readout of constitutive NF-κB transcription. Values are expressed as the mean±s.e.m. of five separate experiments, ^*P<0.05, ^**P<0.01.