Figure 5 | British Journal of Cancer

Figure 5

From: Inhibition of constitutive and cxc-chemokine-induced NF-κB activity potentiates ansamycin-based HSP90-inhibitor cytotoxicity in castrate-resistant prostate cancer cells

Figure 5

Interleukin-8 induces Hsp90 gene transcription in CRPC cells. (A) Bar graphs representing real-time PCR analysis of Hsp90 mRNA transcript levels in PC3 (left panel) and DU145 (right panel) cells after stimulation with 3 nM rh-IL-8 for the indicated times. Values are expressed as the mean±s.e.m. of four and five independent experiments, respectively; *P<0.05; **P<0.01; ***P<0.001. (B and C) Bar graphs illustrating qPCR data for Hsp90 transcript levels in PC3 cells (left panels) and DU145 cells (right panels). Cells were pre-treated for 4 h with either the CXCR2 antagonist AZ10397767 at a final concentration of 20 nM (B) or BAY-11-7082 at a final concentration of 5 μ M (C), before stimulation with 3 nM rh-IL-8 for 24 h. Data shown are shown as mean±s.e.m., calculated from six independent experiments, *P<0.05; **P<0.01; ***P<0.001.

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