Figure 6 | British Journal of Cancer

Figure 6

From: Inhibition of constitutive and cxc-chemokine-induced NF-κB activity potentiates ansamycin-based HSP90-inhibitor cytotoxicity in castrate-resistant prostate cancer cells

Figure 6

Interleukin-8 signalling regulates Hsp90 expression in DU145 cells. Immunoblots illustrating the expression of Hsp90 in cell lysates extracted from (A) PC3 and (B) DU145 cells stimulated with 3 nM rh-IL-8 for indicated times. Equal protein loading was confirmed by re-probing the membranes for GAPDH. (C) Immunoblot detecting levels of Hsp90 expression in the absence and presence of the CXCR2 inhibitor AZ10397767 (20 nM) and BAY11-7082 (5 μ M), administered to both PC3 and DU145 cells for 16 h. (D) Bar graph presenting densitometry analysis of the immunoblots presented in (C) above. The ratio of Hsp90/GAPDH expression is presented as a percentage of the ratio detected in controls.

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