Figure 3

Effect of interleukin-6 (IL-6) overexpression on the production of prostate-specific antigen (PSA) by LNCaP sublines. (A) Enzyme-linked immunosorbent assay (ELISA) was performed in triplicate to analyse the concentrations of PSA protein in culture supernatant from LNCaP sublines cultured in both standard medium and steroid hormone-depleted charcoal-stripped medium for 24 h. Bars, s.d. * differs from LNCaP/Co (P<0.01). (B) ELISA was performed in triplicate to measure serum PSA levels in nude mice bearing subcutaneous tumours of LNCaP sublines before and 2 months after castration. Bars, s.d. of serum PSA; 10 mice per group. * differs from LNCaP/Co (P<0.01).