Figure 3
From: Activation and clinical significance of the unfolded protein response in breast cancer
Induction of GRP78 and XBP1 by glucose deprivation and oestrogen stimulation. Western blots of breast cancer cell line lysates (T47D) after exposure to varying culture conditions to demonstrate GRP78 or XBP1 expression. (A) Cells cultured in differing glucose concentrations varying from normal to 0.2 mM showing progressively increasing GRP78 levels as glucose concentrations fall. (B) Cells cultured in progressively increasing oestrogen concentrations showing GRP78 over-expression. (C) Cells cultured in varying glucose or oestrogen concentrations showed that lack of glucose or oestrogen stimulation induced XBP1 expression.
