Figure 4
From: Activation and clinical significance of the unfolded protein response in breast cancer
The effect of tamoxifen on T47D cells cultured in oestrogen-supplemented media. Western blots of cancer cell line (T47D) lysates after culture under different conditions. (A) Lane plan: 1: standard culture conditions; 2: 0.1% DMSO-supplemented media; 3: 1 nM oestrogen-supplemented media; 4: 1 nM oestrogen plus 1 μ M tamoxifen-supplemented media; 5: 1 nM oestrogen plus 5 μ M tamoxifen-supplemented media; 6: 1 nM oestrogen plus 10 μ M tamoxifen-supplemented media; 7: 1 nM oestrogen plus 15 μ M tamoxifen-supplemented media; 8: 1 nM oestrogen plus 20 μ M tamoxifen-supplemented media; (B) lane plan: 1: standard culture conditions; 2: standard conditions + 0.1% DMSO; 3: 1 μ M tamoxifen-supplemented media; 4: 5 μ M tamoxifen-supplemented media; 5: 10 μ M tamoxifen-supplemented media; 6: 15 μ M tamoxifen-supplemented media; 7: 20 μ M tamoxifen-supplemented media.
