Table 2 HER2/neu expression in primary USPC cell lines

From: In vitro activity of pertuzumab in combination with trastuzumab in uterine serous papillary adenocarcinoma

 

IHC

FISH

RT–PCR

Sample

Tissue

Cell Block

Tissue

Cell Block

mRNA copy number

Control

    

1

 USPC ARK-1

3+

3+

3.1

2.5

373

 USPC ARK-2

3+

3+

6.3

5.2

607

 USPC ARK-3

2+

3+

N/A

4.7

677

 USPC ARK-4

1+

0

1.7

1.6

7

 USPC ARK-5

1+

0

1.5

1.4

13

 USPC ARK-6

1+

1+

N/A

0.9

6

  1. Abbreviations: FISH=fluorescent in situ hybridisation; IHC=immunohistochemistry; RT–PCR=real-time PCR; N/A=not available for testing; USPC=uterine serous papillary adenocarcinoma.
  2. Tissue refers to formalin-fixed, paraffin-embedded tissue blocks of the original tumour sample. Cell Block refers to formalin-fixed, paraffin-embedded cell blocks obtained from primary cell lines in culture.
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