Figure 3
Antibodies-dependent cellular cytotoxicity (ADCC) mediated by human recombinant anti-TPO aAbs (B4 and B4′) and patient's sera anti-TPO aAbs. (A and B) Binding of TPO/anti-TPO complexes on effector cells (HL-60 cell line and PBMC) was analysed by flow cytometry. (C) Antibodies-dependent cellular cytotoxicity was determined by the 51Cr release assay. Human thyroid cells were incubated with irrelevant Abs (□) or human anti-TPO aAbs purified from sera of patients suffering from AITD (
), B4 (
) and B4′ (
) with different effector cells (PBMC and HL-60). Y axis presents the percentage of Jurkat (
) or NPA cells (
) lysis. Bars, s.d. Results are mean of triplicates.
