Figure 4
Complement-dependent cytotoxicity mediated by human recombinants (B4 and B4′) and patients' sera anti-TPO aAbs. (A) The C1q component of Complement was able to bind to TPO/anti-TPO aAbs complexes but not to an irrelevant IgG. Binding was determined by ELISA. Results are mean of duplicates. (B) Expression of complement regulatory proteins CD46, CD55 and CD59 was investigated on NPA cells, by flow cytometry and the data presented in histograms were overlaid. (C) Complement-dependant cytotoxicity was determined by the 51Cr release assay. Y axis presents the percentage of NPA cells lysis. NPA cells were lysed by the complement in presence of human (C1) or murin anti-TPO aAbs (C2). Bars, s.d. Results are means of duplicates.
