Figure 3

Supernatants from tumours treated with chemotherapy stimulate dendritic cells (DCs). DC-derived monocytes were cultured with exhausted culture media from A549 and MCF7 treated cells. The geometric mean fluorescence intensities (GMFIs) and percentages (percentage of cells) of expressions of the maturation markers CD80, CD83 and CD86 were assessed after 24 h by flow cytometry. Representative density plots with %cells distribution within each quadrant (A) and histograms highlighting the GMFI within the defined range (B) are shown for DCs exposed to A549-derived media. These media were from cultures treated with cyclophosphamide (CPM), oxaliplatin (OXP) or gemcitabine (GEM). Tumour-free basal medium (BM) and media from untreated tumour cells (control) were also included. (C) There were generally increases in the markers (either in terms of GMFI and/or percentage of cells) upon exposure to supernatants from cyclophosphamide (C), oxaliplatin (O) or gemcitabine (G) compared with untreated tumours (0), which was most clear in the A549 cohort of samples. Each column is a representative of the mean of at least three separate experiments and s.d. has been omitted for clarity.