Figure 6

(A) Flow cytometry analysis of TUNEL staining in P31 cells after 72 h incubation with 5 mg l^–1 cisplatin and 0.1 μg l^–1 VT-1, alone or in combination. Green dots indicate unstained cells and red TUNEL-stained cells. The percentage mean±s.d. (n=3) of TUNEL-stained cells is noted in the dot plots. (B) Flow cytometry analysis of Gb3 expression in P31 and P31res cells after 72 h incubation with 2 μmol l^–1 PPMP. The percentage mean±s.d. (n=3) of Gb3-stained cells is noted in each dot plot. (C) Flow cytometry analysis of TUNEL staining in P31and P31res cells pre-incubated with 2 μmol l^–1 PPMP, and after 72 h incubation with 5 mg l^–1 cisplatin and 0.1 μg l^–1 VT-1, alone or in combination. Green dots indicate unstained cells and red TUNEL-stained cells. The percentage mean±s.d. (n=3) of TUNEL-stained cells is noted in each dot plot. Representative results are shown in A–C.