Figure 1
From: Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1
The effects of Id-1 overexpression in LNCaP prostate cancer cells in prostate cancer cell-mediated bone cell activities. Three independent clones of Id-1 overexpressing cells previously generated were used in this study. (A) Western blot analysis shows increased Id-1 protein expression compared with vector control. (B) Alizarin red S assay; conditioned medium from LNCaP-Id-1 clones inhibited mineralisation of SaOS-2 osteosarcoma cells, whereas conditioned medium from LNCaP-vector control cells stimulated mineralisation of SaOS-2 osteosarcoma cells when compared with control. (C) Quantitative analysis of the extent of mineralisation. (D) Conditioned medium from LNCaP-Id-1 clones stimulated the differentiation of RAW264.7 cells to osteoclast-like cells, whereas medium from LNCaP-vector control conferred no significant effect when compared with control. Representative area under × 200 magnification. (E) Quantitative analysis of the osteoclast differentiation assay. Columns represent the mean values from three independent experiments±s.d.
