Figure 2
From: Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1
The effects of Id-1 knockdown on PC3-mediated bone cell activities. (A) PC3 shId-1 cells were generated by lentiviral transduction and the reduction in Id-1 expression was confirmed using western blot analysis. (B) Conditioned media from both PC3 shCon and shId-1 cells conferred inhibitory effects on SaOS-2 mineralisation, indicating that knockdown of Id-1 is not sufficient to modulate the ability of PC3 cells to stimulate osteoblast mineralisation. (C) Conditioned medium from PC3 shId-1 cells had a lower ability to stimulate osteoclast differentiation when compared with that from PC3 shCon cells. (D) Quantitative analysis of osteoclast differentiation in PC3. Columns represent the mean values from three independent experiments±s.d.
