Figure 3
From: Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1
Id-1 negatively regulates TNF-β expression. (A) Using RT–PCR, we found that the expression of TNF-β mRNA was lower in Id-1 overexpressing LNCaP cells and was higher in Id-1 knockdown PC3 cells compared with respective controls. Water was used in place of template in the negative control. (B) Id-1 negatively regulated the promoter activity of TNF-β, suggesting that the regulation might be at the transcriptional level. (C) Western blot showed similar results at the protein level. Columns represent the mean values from three independent experiments±s.d.
