Figure 5
From: Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1
Recombinant TNF-β protein rescued the effect of Id-1 overexpression in prostate cancer-mediated osteoblast mineralisation. (A) Alizarin red S assay shows that TNF-β partially reversed the inhibitory effect of overexpressing Id-1 in LNCaP-mediated osteoblast mineralisation. (B) Quantitative analysis of the extent of mineralisation in alizarin red S assay. Light and dark grey columns in bar chart represent results from conditioned medium with and without the addition of TNF-β recombinant protein, respectively. Columns represent the mean values from three independent experiments±s.d.
