Figure 4 | British Journal of Cancer

Figure 4

From: Role of reactive oxygen species in brucein D-mediated p38-mitogen-activated protein kinase and nuclear factor-κB signalling pathways in human pancreatic adenocarcinoma cells

Figure 4

Effects of pre-treatment with NAC of the phosphorylation of p38-MAPK after treatment with BD. Cells were pre-treated with 250 μ M NAC for 1 h, then incubated with 30 μ M BD for 15 min to 2 h. (A) Western bolt analysis of samples using total p38 (p38-MAPK) and phosphorylation-specific p38 (p-p38-MAPK) antibodies. The results were obtained from three independent experiments. (B) Quantitative analysis of western blot band intensities. *P<0.05 vs vehicle control, **P<0.01 vs control and ##P<0.01 vs 30 μ M BD treatment.

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