Figure 6

Brucein D-induced inhibition of NF-κB activation is independent of ROS in PANC-1 cells. Cells were pre-treated with 250 μ M NAC or vehicle for 1 h, then incubated with 30 μ M BD for 15 min to 2 h. Western blot analysis was performed on cytoplasmic lysate using anti-phospho-IκBα antibodies (A) and nuclear lysate using anti-phospho-NF-κB p65 antibodies (B). ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs vehicle control. The results were obtained from three independent experiments. Quantitative analysis of band intensities is shown in lower panels, respectively. (C) Representative image showing the results from EMSA of nuclear extracts of PANC-1 cells after BD treatment for 2 h. Lane 1: distilled water (negative control); Lane 2: HeLa cell extract (positive control); Lane 3: vehicle control; Lane 4: 250 μ M NAC treatment; Lane 5: 30 μ M BD treatment; Lane 6: 250 μ M NAC + 30 μ M BD treatment. Similar results were obtained in three independent experiments.