Table 1 Immunohistochemical staining procedures

From: Expression of aldehyde dehydrogenase 1 (ALDH1) is associated with basal-like markers and features of aggressive tumours in African breast cancer

Biomarker

Antibody

Clone

Dilution

Incubation time (min)

ER

MMA oestrogen receptor-α

1D5

1 : 50

30

PR

MMA progesterone receptor

PgR 636

1 : 150

30

HER2

RA c-erbB-2 oncoprotein

Polyclonal

1 : 500

60

Ki-67

MMA Ki-67 antigen

MIB-1

1 : 50

60

p53

MMA p53 protein

DO-7

1 : 1000

60

p63

MMA p63 protein

A4A

1 : 300

30

CK 5/6

MMA cytokeratin 5/6

D5/16 B4

1 : 200

30

P-cadherin

P-cadherin purified MA

56

1 : 400

60

EGFR

Mouse anti-EGFR

31G7

1 : 30

30

c-kit

RA CD117, c-kit

Polyclonal

1 : 200

30

ALDH1

Purified M anti-ALDH

44

1 : 250

60

BMI-1

Anti-BMI-1

6C9

1 : 1

60

  1. Abbreviations: MMA=monoclonal mouse antihuman; MA=mouse antihuman; RA=rabbit antihuman; M=mouse; ER=oestrogen receptor; PR=progesterone receptor; EGFR=epidermal growth factor receptor; ALDH1=aldehyde dehydrogenase 1; CK=cytokeratin.
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