Figure 3
Comparison of transactivation potential by EWS-Oct-4B. Cells (293T) were cotransfected with expression vectors encoding the indicated amounts of EWS-Oct-4 (grey bars) or EWS-Oct-4B (black bars), the pOct-4(10 × ) TATA luc reporter plasmid, and the Renilla luciferase (top panel). Reporter activity was normalised with Renilla luciferase activity to correct for different transfection efficiencies. Fold induction is expressed relative to the empty expression vector. Each transfection was performed at least thrice independently and the mean values are plotted with their standard errors (±s.e., vertical bars). Extracts for luciferase assays were resolved using 12% SDS–PAGE, transferred to a PVDF membrane, and immunoblotted with anti-Oct-4 (C-20) (middle panel) or anti-EGFP (bottom panel) antibodies, as indicated.
