Figure 8
Characterisation of the effects of ZHBTc ES cells expressing EWS-Oct-4B in nude mice. (A) Effects of EWS-Oct-4B expression on the development of tumours in nude mice. Approximately 0.6 × 107 Oct-4-null ZHBTc4 ES cells expressing EWS-Oct-4B-EGFP or EGFP, were suspended in 100 μl PBS and injected into 5-week-old Balb/c athymic nude mice. Tumour development was observed and photographs were taken 26 days after injection. (B) Weight of tumours from nude mice injected with Oct-4-null ZHBTc ES cells expressing EWS-Oct-4B. Tumour weight in individual animals was measured at 26 days after injection (n=6) and is plotted as mean increases±s.e.
