Figure 2

Inhibition of migration of carcinoma cells by EG3287 and NRP1 silencing. (A, D) A549 and ACHN cells were pretreated for 30 min with the indicated concentrations of EG3287 and chemotaxis towards 0, 0.5, or 1% FBS was determined after 4 h of incubation. ^*P<0.05; ^**P<0.01; and ^***P<0.001 vs untreated control. (B, E) A549 and ACHN cells were transfected with NeoFX only as a mock control (M) or siRNAs, as indicated, and total cellular protein was extracted 48 h after transfection, and NRP1 and NRP2 were detected by immunobloting. GAPDH served as loading control. (C, F) siRNA-transfected A549 and ACHN cells were loaded into the top inserts of transwell plates and chemotaxis towards 0, 0.5, or 1% FBS was determined after 4 h of incubation. ^*P<0.05 vs control siRNA.