Figure 3

Suppression of cell–matrix adhesion and enhancement of the inhibitory effects of an integrin-β1-function-blocking antibody by EG3287. (A, C) A549 and ACHN cells were pretreated for 30 min with the indicated concentrations of EG3287 and seeded to wells coated with the indicated matrix proteins. After 90 min of incubation, attached cells were labelled with calcein-AM and measured using a fluorescence plate reader. ^*P<0.05; ^**P<0.01 vs untreated control. (B, D) A549 and ACHN cells were pretreated for 30 min with an integrin-β1-blocking antibody at the indicated concentrations in the absence or presence of 100 μ M EG3287, and cell adhesion to matrix proteins was measured. ^*P<0.05; ^**P<0.01 for integrin-β1 antibody alone vs integrin-β1 antibody plus EG3287.