Figure 3
Ki26894 inhibits invasion by scirrhous gastric cancer cells. (A) Representative pictures of invading gastric cancer cells. OCUM-12 cells invaded into a 12 μm-pore membrane filter in the presence of TGF-β1 when compared with control. Most of the invaded cells show epithelial-to-mesenchymal transition (arrow heads). Ki26894 (10 μ M) inhibited the invasion induced by TGF-β1. (B) Transforming growth factor-β1 significantly stimulated the invasive behaviour of OCUM-2MLN and OCUM-12 cells. Ki26894 (10 μ M) significantly inhibited the invasion seen in these cells. In contrast, invasion by non-scirrhous gastric cancer cell lines, MKN-45 and MKN-74, was not affected by TGF-β1 or by Ki26894.
