Figure 5
Effects of Ki26894 on cellular migration signals in gastric cancer cells. (A) ELISA of RhoA. Transforming growth factor-β1 (10 ng ml−1) significantly upregulated the active form of RhoA in scirrhous gastric cancer cell lines, OCUM-2MLN and OCUM-12, and Ki26894 (10 μ M) significantly inhibited this effect. In contrast, the active form of RhoA was not increased by TGF-β1 or by Ki26894 in non-scirrhous cancer cell lines, MKN-45 and MKN-74. (B) Transforming growth factor-β1 (10 ng ml−1) increased myosin light chain-2 phosphorylation (p-myosin) and decreased ZO-2 and E-cadherin expression in scirrhous gastric cancer cell lines, but not in non-scirrhous gastric cancer cell lines. Ki26894 (10 μ M) decreased p-myosin, and increased ZO-2 and E-cadherin expression in scirrhous gastric cancer cell lines.
