Table 1 Characteristics of the different selected housekeeping genes
From: ‘Desperate house genes’: the dramatic example of hypoxia
Gene name | Abbreviation | Function | Accession IDs | 5′-Primers-3′ (forward/reverse) | Size (bp) |
|---|---|---|---|---|---|
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9) | ATP5G3 | Oxydative phosphorylation | GGATTTGCCTTGTCTGAAGC CGTACATTCCCATGACACCA | 188 | |
β-2-Microglobulin | B2M | β-Chain of major histocompatibility complex class 1 molecules | CTCACGTCATCCAGCAGAGA TCTTTTTCAGTGGGGGTGAA | 198 | |
β-Actin | ACTB | Cytoskeletal structural protein | GGGGTGTTGAAGGTCTCAAA GGCATCCTCACCCTGAAGTA | 183 | |
β-Glucuronidase | GUSB | Exoglycosidase in lysosomes | CTGTACACGACACCCACCAC ATTCGCCACGACTTTGTT | 208 | |
Cyclophilin A | PPIA | Serine–threonine phosphatase inhibitor | ACCGTGTTCTTCGACATTGC GGCATGAATATTGTGGAGGC | 410 | |
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | Glycolysis enzyme | GAGTCCACTGGCGTCTTCAC GGTGCTAAGCAGTTGGTGGT | 177 | |
Hypoxanthine phosphoribosyltransferase 1 | HPRT1 | Hydrolase in carbohydrate metabolism | TGCTCGAGATGTGATGAAGG TCCCCTGTTGACTGGTCATT | 181 | |
Phosphoglycerate kinase 1 | PGK1 | Glycolysis enzyme | GAAGTGGAGAAAGCCTGTGC CTCTGTGAGCAGTGCCAAAA | 159 | |
TATA-box-binding protein | TBP | General RNA polymerase II transcription factor | TGCCTCCAGAATATGCCTCT CAATGGTTTTCAAGCTTTCCA | 203 | |
Transferrin receptor (p90, CD71) | TFRC | Cellular iron uptake | GGAGAATCCTGGGGGTTATG GCTTTCAGCATTTGCAACCT | 202 |
