Figure 4

Impact of fibroblast growth factor receptor 3c (FGFR3c) knockdown on growth and viability. (A) Small interfering RNA (siRNA) directed against FGFR3-IIIc was introduced into SW480 cells by lipofection, and RNA (left panel) and protein (right panel) were assessed 48 h later. (B–D) For determination of growth and viability, cells were plated in 96-well plates at 2 × 10³ cells per well and siRNA was delivered twice 24 and 48 h later. After 48 h, cell viability (B) and induction of apoptosis (C) and DNA synthesis (D) were determined as described in the Materials and methods section. ^*, ^** and #, ## indicate increase or decrease, respectively, as compared with control at P<0.05 or P<0.01 (Student's t-test).