Figure 8
From: Fibroblast growth factor receptor 3-IIIc mediates colorectal cancer growth and migration
Fibroblast growth factor receptor 3-IIIc (FGFR3-IIIc)-dependent tumour cell migration. (A) FGFR3-IIIc-dependent signalling was inhibited by infection with dominant-negative adenoviral constructs in SW480 (left panel) and Caco2 cells (right panel). Cells were seeded into filter inserts at 0.5 × 105 cells per well and kept in 1% serum for 24 h. Cell migration was determined by staining cells at the bottom of the membrane or in the lower chamber with crystal violet. (B) Stable FGFR3 transfectants were grown to confluence in six-well plates and scratch wounded. Migration was determined by measuring scratch closure over time. Data points represent the distance migrated by each culture. All data points are calculated as the mean±s.e.m. from four cultures, with ** and # indicating an increase or decrease as compared with control at P<0.05 (Kruskal–Wallis test). NS, not significant.
