Figure 3 | British Journal of Cancer

Figure 3

From: Expansion of CD133+ colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery

Figure 3

Expression of stem cell markers in colon tumour spheroid cells and their downregulation after differentiation. (A) NES, BMI-1, and MSI-1 are detected by immunofluorescence staining in spheroid populations (upper panel). After differentiation, the numbers of cells expressing these stem cell markers reduced significantly (lower panel). (B) Decreases in the number of NES-, BMI-1-, and MSI-1-expressing cells after differentiation were quantified by manual cell counting. (C) Quantitative reverse transcriptase (RT) PCR studies reveal a decrease in the expression levels of ABCG2, but not ABCC1 and ABC1 genes, after differentiation. **P<0.01.

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