Table 3 Confirmation of microarray results with RT-qPCR

From: Adipose tissue pathways involved in weight loss of cancer cachexia

 

Array cachexia/control a

RT-qPCR cachexia/control a

P -value b

Array lean/obese c

RT-qPCR lean/obese d

P -value b

Cell(1)/SVF(0) e

Cell adhesion

 ITGB5

0.76

0.70

0.00021

0.62

0.65

0.0001

0

 LOXL2

0.69

0.58

0.031

0.79

0.69

0.068

1

 MFAP4

0.68

0.63

0.0089

0.57

0.65

0.01

0

 ACTN1

0.77

0.55

0.0014

0.77

0.81

0.02

1

 ECM2

0.78

0.64

0.0031

 

1.04

0.37

1

 DPT

0.64

0.46

0.0013

 

1.04

0.42

1

Extracellular matrix

 LTBP2f

0.69

0.54

0.000044

0.54

0.36

0.0003

0

 FBLN1

0.69

0.60

0.0042

 

0.75

0.085

0

 CILP

0.63

0.66

0.028

 

0.33

0.0031

0

Actin cytoskeleton

 SPTAN1

0.71

0.64

0.0018

0.85

0.83

0.12

0

Mitochondrion

 COX8af

1.22

1.50

0.0079

1.22

1.00

0.49

1

 CYC1

1.24

1.40

0.0076

1.21

1.06

0.30

1

 PC

1.45

1.90

0.00027

1.38

1.42

0.0017

1

  1. Abbreviation: RT-qPCR=real-time quantitative PCR.
  2. a Cachexia (n=13), control (n=14).
  3. bt-test, one-sided.
  4. c Non-obese (n=17) and 20 obese (n=20).
  5. d Non-obese (n=13) and 20 obese (n=19).
  6. e Genes enriched in fat cells (1) or the stroma vascular fraction (SVF) (0) of white adipose tissue.
  7. f Significant difference on microarrays with FDR 1% between cachectic and weight-stable patients. Remaining genes are significant with an FDR of 5%.
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