Figure 2

The CPK method isolates a highly enriched population of CTCs. Percentage of total cells captured by the CPK method from patients with NSCLC, staining for cytokeratin (AE1/AE3), CD45, or DAPI nuclear stain alone by (A) immunofluorescence or (B) FACS. Similar results were seen with samples from patients with breast cancer. (C) Representative immunofluorescence image of CPK-captured cells from patient with NSCLC, labelled with cytokeratin (green), DAPI (blue), and CD45 (red).